study guide Chapters 13 and 24

Note:  We are not doing Chapter 14 at all!

Chapter 13 Cell Cycle

13.1 Overview of the cell cycle

Model systems: Xenopus, Saccharomyces cerevisiae and Schizosaccharomyces pombe

Temperature sensitive mutants

Figures 13-2 and 13-4

13.2 Biochemical studies

Evidence for Maturation-promoting factor (MPF)

 Figures 13-5*, 13-7, 13-8, 13-9 

13.3 Genetic studies with S. pombe

This is the terminology I'll use in class!

cdc2, cdc13, Wee, cdc25, CAK

cyclin and the cyclin dependent kinase

Figures 13-10, 13-11, 13-12, 13-13*

13.4 Mitotic events

Lamin and control of lamin dissociation

Figures 13-15, 13-18, 13-21

13.5 S. cerevisiae

START and the controlling factors

Association of cdc28 with Cln1, Cln2 and Cln3

Figures13-22, 13-23, 13-24, 13-26

13.6 Mammalian cycle

Not much, just look at cycle and Rb Figures 13-29, 13-31

13.7  checkpoints

only p53 - pages 531-532

Question 1-8, 10

Chapter 24 Cancer

24.1 Tumor cells and the onset of cancer

Benign, invasive, malignant

Cell cultures normal versus transformed cells

Changes in cultures cells

Figures 24-3, 24-4, 24-6

24.2  Proto-oncogenes, Tumor-suppressor genes

Types of proteins that control cell growth Figure 24-9*

Retinoblastoma Figures 24-11, 24-12

24.3 Oncogenic mutations

Cell surface receptor mutations Figure 24-15*

24.4 Loss of cell cycle control

Again look at Rb page 1075, Figure 24-19

24.5 Genome stability 

p53 pages 1076-1078, Figure 24-21

Questions 1-8

Old  Material

Look over old study guides! Particularly the figures with asterisks for ALL chapters. There will be a pH question, at one least eukaryotic gene organization question, a protein targeting question like the one on exam 2, an alternative splicing question and a questions utilizing protein and RNA gel data.  Know about Ras, p53 and Rb.  Know what temperature sensitive mutations can tell you.

The first part of the book (chapters 1-8) are the groundwork There will be only a few questions directly on these chapters but you will need to be familiar with those materials to do well on the final. Obviously chapters 11, 13 and 24 will be emphasized although we covered only sections of these chapters. 

Look over the keywords for the new chapters and the older emphasized chapters. 

So the new chapters are  11, 13 and 24

For the rest of the book:

Very important chapters: 17, 18, 19, and 20

Medium chapters: 15, 9, 10

Less emphasized chapters: 1-8

Not covered at all: 6, 12, 14, 16, 21, 22, and 23