7.1  DNA cloning and plasmid vectors

Review Plasmids, recombinant plasmids

restriction enzymes

Figures 7-1, 7-3, 7-4, 7-5, 7-7,  Table 7-1

7.2 DNA libraries

lambda phage

construction of genomic DNA

Isolation of mRNA, construction of cDNA library

cosmid vectors – size of insert

Figures  7-10, 7-11, 7-12, 7-14, 7-15,  Table 7-2

7.3  Identifying and analyzing cloned DNA

identification of a clone – how do you find the needle in the haystack?

Making a specific probe for the gene of a protein

DNA sequencing

Figures  7-17, 7-18, 7-19, 7-22, 7-23 DNA gel, 7-28, 7-29

7.4 Bioinformatics

The organization of genes in eukaryotic organisms

Figure  7-31, Table 7-3

7.5 Analyzing specific genes in complex mixtures

Southern blots and Northern blots

Polymerase chain reaction (PCR)

Figures  7-31, 7-32, 7-33

7.6 nothing

7.7 Polymerase Chain reaction

Figure 7-38

7.8 nothing

Questions 1, 2, 4, 6, 7, 8-10

Chapter 8

8.1 Genotype and phenotype

Mutants are dominant or recessive

types of DNA changes – Figure 8-4

From notes – sickle cell anemia example see also page 258-259

Figure 8-4*, 8-5*, 8-7

8.2  Cell cycle mutants, temperature sensitive mutants

complementation tests

Figures 8-8, 8-9*, 8-11

8.3  Cell cycle mutants, secretion mutants

RFLP - Figure 8-20*

8.4  nothing

8.5  Transgenes

Knock-out experiments in mice

Cystic fibrosis – page 287

Figures 8-30, 8-31, 8-34

Questions 6-9, 10-13

5.1 Microscopy

Light microscopy and its limits; Phase contrast, fluorescence microscopy

Electron microscopy – what are the advantages and limitations?

Figures 5-2, 5-3, 5-5, 5-6, 5-9, 5-12, 5-15, 5-16, 5-17*, 5-18

5.2 Purification of cell parts

Differential centrifugation, equilibrium densisty centrifugation

Figures 5-3, 5-24

Don’t worry about flow-cytometry

5.3 Biomembranes

Review biomembranes of eukaryotic cell (pages 157-168

composition of membranes

Figures  5-27, 5-28, 5-30, 5-31, 5-35, 5-36, 5-42

5.4 Organelles of the eukaryotic cell

Review parts of the cell (organelles) pages 168-176

Figures  5-43, 5-44, 5-45, 5-46, 5-47, 5-49

Questions 1, 2, 5-8, 10-13