Sample Questions Exam I, BIOL 3090

 

  1. Which amino acid has a positive charge at neutral pH?

    2. a). glycine b). lysine c). proline d). alanine e). glutamic acid

  2. Name two hydrophobic amino acids
  3. Which amino acid sequence would most likely be part of a transmembrane helix?

a). ALIFLVAAGVLIW

b). ALEDLVAARELIW

c). ALPFLVAPGVLPW

4. Which of the following would be a conservative substitution in a protein?

a). serine for threonine

b). lysine for glutamic acid

c). lysine for proline

  1. Write the sequence of the DNA strand that would complement the sequence: 5’ A-G-C-C-T-A-G-C-T-T 3’

6. Write the RNA that would be made from the sequence:

5’ G-G-T-T-C-A-A 3’

  1. What process RNA polymerase does catalyze?

    2. a). protein synthesis

    b). translation

    c). transcription

    d). replication

    e). subscription

  2. Where would you expect a m7G? Be as specific as possible!
  3. Where would you find a Shine-Dalgarno sequence?

10. Which organelle(s) contains DNA?

a). nucleus

b). mitochondria

c). chloroplasts

d). all of the above

e). none of the above

11. What method would you use to separate two proteins, one that has a molecular mass of 100 kDa and one with a mass of 15 kDa?

12. If you had antibodies how would you detect the presence of a protein in a whole cell extract?

  1. If you could change the anticodon of a specific tRNA from 5’ A-C-U 3’ to 5’ A-C-G 3’ and it still bound the same amino acid how would the protein sequence change? Note: it may not change.
  2. Write the general structure of a nucleotide.
  3. If the adenine content of DNA from an organism is 36% what is the guanine content?
  4. Where would you expect to find enzymes that degrade proteins and lipids in an eukaryotic cell?

    5. a). Mitochondria

    b). Endoplasmic reticulum

    c). Golgi bodies

    d). Lysosome

    e). Peroxisome

  5. Give the sequence of a mRNA that encodes for the amino acid sequence A-D-K-Y.
  6. Give the amino acid sequence of the peptide encoded by an mRNA with the sequence C-A-C-U-C-C-A-A-U-G-G-U
  7. The DNA sequence is 5’ A-G-G-C-T-A-C-G-C-T-A-G 3’. What peptide sequence will this encode?

Answers

 

1. B

2. Leucine, isoleucine, valine, phenylalanine, tryptophan

3. A

4. A

5. 5' A-A-G-C-T-A-G-G-C-T 3'

6. 5' U-U-G-A-A-C-C- 3'

7. C

8. 5' end of a eukaryotic mRNA

9. In the 5' UTR of a bacterial mRNA

10. D

11. Column chromatography or SDS polyacrylamide gel electrophoresis.  Either method should work.

12.  using a western blot

13.  serine would be put into the protein in place of arginine whenever the 5' C-G-U 3' codon occurred in mRNA

14.  phosphate -sugar - base

15.  G = 14% (A+T, G+C, A+T+G+C =100%)

16.  lysosome

17. 5' G-C-C-G-A-U-A-A-A-U-A-U 3'

18. His - Ser - Asn - Gly (or HSNG)

19. Leu - Ala - stop

Answers to assigned questions in the book

Chapters 1-5, 7&8

Chapter 1

No questions assigned

Chapter 2

  1. A;   2. C;  3. D;  4. B;  5. B;   6. B;  7. A

  Chapter 3.

  1.      B;  2. D;  3. A;  5. B;  6. D;  7. C;  8. C;  10. C;  11. A;  12. C

  C hapter 4.

  1.      D;  2. B;  4. A

  Chapter 5

  1.      C;  2. B;  5. C;  6. C;  7. A;  8. B;  10. D;  11. C;  12. B;  13. D

  Chapter 7

  1.      A;  2. B;  4. A;  6. B;  7. C;  8. B;  9. D;  10. D

  Chapter 8

  6. A;  7. D;  8. A;  9. D;  10. D;  11. B;  12. B;  13. B