Sample questions Exam III BIOL 3090

1. peptide hormones bind to receptors located in the

a). plasma membrane

b). cytoplasm

c). nucleus

d). mitochondria

2.  Which statement below best describes the structures in a centriole?

   a). they are singlet microtubules

   b). they are doublet microtubules

   c). they are  triplet microtubules

   d). they are mono-microfilaments

   e). they are double-microfilaments 

3. What best describes a “dual specificity kinase”? Identify the best answer.

  a). It can phosphorylate two different proteins.

  b). It can activate two different pathways.

  c). It can activate one pathway while at the same time inhibit another pathway.

  d). Two different G-proteins can activate this kinase.

  e). It can phosphorylate tyrosine residues and threonine and serine residues.

4. What is a retrotransposon?

5. Name a protein kinase associated with the Ras-independent insulin signal transduction pathway

6. In RTK signaling which best describes GRB2?

a). enzyme

b). receptor

c). G-protein

d). adapter

7. Ras is a _________.

8. The insulin regulated glucose transporter is:

a). GLUT1

b). GLUT2

c). GLUT 3

d). GLUT4

e). GLUT5

9. What does a SH2 domain bind to?

10. Microtubules are composed of

a. Alpha tubulin

b. Beta tubulin

c. Gamma tubulin

d. Alpha and beta

e. Alpha, beta and gamma

11. Tau protein is an example of a

a). MTOC

b). G-protein

c). (-) directed motor

d). MAP

12. Which end of the microtubule will a kinesin-coated vesicle be taken?

13. How many microtubules are in a flagella?

14. If a mutation caused GRB2 to bind more tightly to Sos in the absence of an RTK do you think Ras will be more active or less active?

15. What domain of the GRB2 protein in question 14 would you expect to find the mutation?

16. Vesicles in axons have both myosin and kinesin bound to them. Why?
17. Which end of the microtubule are kinetochores attached?

18. How does the kinetochore move towards the poles?

a). dynein motors

b). kinesin motors

c). tau interactions

d). microtubule depolymerization

19. How are the components of the cytoskeleton in figure 19-50 visualized?

20. In eukaryotes is the poly A tail added to the last nucleotide added by RNA polymerase or is some other process taking place in addition?

ANSWERS

Answers to questions in the chapters.

Chapter 19. 5. A, 6. D, 7. C, 8. D, 9. B, 11. C, 12. C, 13. D, 14. A

Chapter 20. 6. A, 7. D, 8. C, 9. A, 10. D, 11. B, 12. C, 13. D

Chapter 9. 2. D, 3. A, 4. D

Chapter 10. 1. C, 2. D, 3. A, 4. D, 5. B, 6. D, 7. A, 9. A, 10. C

Answers to sample questions.

1. A
2. C

3. E

4. A type of eukaryotic mobile DNA element that goes through an RNA intermediate

5. Insulin receptor or Protein kinase B (both answers are correct)

6. D

7. Ras is a GTP binding switch - part of signal transduction pathway

8. D

9. A phosphotyrosine  

10 alpha and beta tubulin

11. D; 

12 the (+) end

13. Nine doublets and two single tubules

14. Hard to say. If it binds better while associated with the RTK then Ras might be more activated. However if it binds Sos in the absence of RTK binding it might inhibit the ability of Sos to activate.

15. SH3 domain

16. Vesicles can travel along microfilaments or microtubules

17. (+) end

18. D

19. immunofluorescence

20. The RNA is first cleaved at the 3'end and then polyadenylation takes place.