You are studying gene Q in yeast, which is an eukaryote but normally grows as a haploid. Protein Q is 50kDa. The restriction map of the wild-type allele of this gene is shown below:

You are studying 4 mutant strains with mutations in this gene. You analyze wild-type and each of the mutants in the following ways:

i) Southern blot: cut genomic DNA isolated from each strain with EcoRI, run on gel, blot, and probe with radioactive 4.8kbp BamHI fragment of gene Q.

ii) Northern blot: run RNA isolated from each strain on gel, blot, and probe with radioactive 4.8kb p BamHI fragment of gene Q.

iii) Western blot: run protein isolated from each strain on gel, blot, and probe with radioactive anti-Q antibodies.

Draw and explain the resulting three blots (i, ii, iii) from the following strains:

a) wild-type.

b) a mutant with a deletion of the region from 0.8kbp to 1.0kbp which partially inactivates the promoter, resulting in 5-fold lower levels of transcription. Assume that the translation rate of Q mRNA molecules (protein molecules per mRNA molecule) is normal.

c) a mutant with a point nonsense mutation at 2.5kbp.

d) a point missense mutant at 2.6kbp that prevents EcoRI from cutting at that site.

e) a deletion of the region from 2.0kbp to 2.5kbp.