November 24

See AFTER EXAM 3 READINGS (Moodle), Chapters 20 - 21

Continue Discussion of Viruses from Lecture 33

Biotechnology

1.

2.

a.

b.

Important Techniques

Recombinant DNA

Genomic Libraries

 

 

DNA Cloning

 

 

 

DNA Amplification in vitro  

 

 

 

Applications of DNA Technology

1.

2.

3.

4.

5.

Genomes and Their Evolution

1.

2.

3.

4.

Genome Sequencing

 

Genome Sizes and Estimated Numbers

Human Genome

Analysis of Genomes

 

 

QUESTIONS (see question 9 from Lecture 33)

1. What is a restriction enzyme and what is the role of restriction enzymes in making recombinant DNA?  What are three kinds of genomic libraries and how do they differ?

2. What is a plasmid?  How can genes of interest be cloned in bacteria using plasmids?  What is the purpose of DNA cloning?

3. What technique is useful for amplifying DNA in vitro?  When is this technique most useful?

4. Give four applications of DNA technology.  Why is DNA technology important for the production of insulin and human growth hormone?  What is the "golden rice" and why was it produced?

5. What are two goals of genome projects?  What are the steps involved in "shotgun" sequencing of genomes?

6. How does the number of genes per MB of genome compare between Homo sapiens and E. coli?  Why are they different?

7. How much of the human genome is made up of genes coding for protein or gives rise to rRNA or tRNA?  What other types of DNA are there in the genome?