Sewell Suon (HHMI EXROP) Isiah Warner, Chemistry
Understanding The Formation Of Atherosclerotic Plaque: Identification Of Glycoproteins Associated With Atherosclerotic Plaque

The main focus is to isolate glycosylated proteins that are in tissue extracts of human aorta, native and bypass arteries. A variety of separation techniques used to analyze proteins in the formation of atherosclerotic plaque include 1D SDS-PAGE, 2D gel electrophoresis, Western Blotting and enzyme immunoassay. The resulting proteins in a gel matrix will be digested using trypsin and further analyzed using matrix assisted laser desorption ionization mass spectrometry (MALDI MS). Further elucidation of the peptide sequences will be analyzed using protein databases such as MASCOT, Protein Prospector, or Swiss Prot. Proteins with limited matches from these databases will be sequenced using Liquid Chromatography Mass Spectrometry (LC-MS / MS). These peptides will go through mass spectrometry analysis for matching and identification.