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2002
LSU-HHMI Summer Undergraduate Research Program |
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 Roosevelt
V Campbell (University of Texas – Pan American) (Mohamed
A. F. Noor, LSU Dept. of Biological Sciences) Elucidation
of Genetic Changes Associated with the Transcription of TRAP100
in Related Species of Drosophila
Thyroid Receptor Associated Protein (TRAP100) gene appears to
be transcribed in two directions in Drosophila pseudoobscura.
Only one of these transcripts is expressed in D. melanogaster
and D. simulans, and the other transcript is underexpressed
in hybrids of D. pseudoobscura and D. persimilis. Both transcripts
are polyadenylated in D. pseudoobscura, and the signals for
polyadenylation are present in D. pseudoobscura but not D. melanogaster
genomic DNA. Here, I have sequenced part of this gene in multiple
lines of D. pseudoobscura and in several other related species
to elucidate the genetic changes that may have produced this
new transcript. I find that the region bearing the polyadenylation
signals is highly variable among Drosophila species, while most
of the remainder of the gene is conserved in sequence.
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