SMC Home > Staff > Matthew
Brown
Matthew Brown, Ph.D.
Research Associate
mbrown7@lsu.edu
(225)578-8246
As light microscopy specialist for the SMC,
I have experience with all of the light microscopes in our facility,
though my specialty is fluorescent microscopy. I have experience
using our Leica TCS SP2 scanning laser confocal microscope in
subcellular localization of proteins in both mammalian cell culture
and unfixed plant plant tissues. I am also well versed in the
use of our Leica RXA widefield fluorescent microscope and the
Slidebook software used for image capture, deconvolution, and
quantitaion on this system. I also have some experience with
brightfield microscopy, including DIC and phase contrast, as
well as a experience imaging from our steromicroscopes. I am
also familiar with advanced image processing techniques using
Adobe Photoshop.
I earned my Ph.D. in Biochemistry from LSU in 2006. I studied
cell fate determination in the epidermis of the leaves of Arabidopsis
thaliana with Dr. John C. Larkin at LSU. The challenges presented
by my Ph.D. studies led to the microscopy experience
I now possess.
I also have experience with molecular genetic techniques, such
as construction of recombinant DNA molecules and quantitative
PCR.
Larkin, J.C., Brown, M.L., and Schiefelbein, J. 2003. “How
do cells know what they want to be when they grow up? Lessons
from epidermal patterning in plants.” Annu. Rev. Plant
Biology 54: 403-430.
Churchman, M.L., Brown, M.L., Kato, N., Kirik, V., Hulskamp,
M., Inze, D., De Veylder, L., Walker, J.D., Zheng, Z., Oppenheimer,
D.G., Gwin, T. Churchman, J., Larkin, J.C. 2006. "SIAMESE, a
plant-specific cell cycle regulator, controls endoreplication
onset in Arabidopsis thaliana." Plant Cell (11) 3145-57.
